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. 2013 Aug 15;123(9):3983–3996. doi: 10.1172/JCI65842

Figure 4. Epithelial hVDR preserves epithelial tight junctions and suppresses colonic inflammation in a TNBS-induced colitis model.

Figure 4

(A) ZO-1 immunostaining (red) in untreated (Ctrl) and TNBS-treated WT and Tg colons on day 3. Arrows indicate the loss of ZO-1 protein in the luminal epithelium in TNBS-treated WT mice. (B) Real-time RT-PCR quantitation of tight junction protein transcripts in control and TNBS-treated WT and Tg colons. (C) Real-time RT-PCR quantitation of proinflammatory cytokines and chemokines in colonic mucosa from control and TNBS-treated WT and Tg mice on day 6. (D) Fold change induction of proinflammatory cytokines and chemokines in purified colonic epithelial cells from TNBS-treated WT and Tg mice at 8 hours and on day 2 compared with purified untreated control epithelial cells. *P < 0.05; **P < 0.01; §P < 0001. n = 5–6 in each genotype.