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. 2013 Aug 15;123(9):4010–4022. doi: 10.1172/JCI69443

Figure 6. Inducible knockdown of SHN3 increases bone mass in adult mice.

Figure 6

(A) 8-week-old WT and Shn3KD mice were fed DOX-containing chow for 6 weeks, and total RNA was extracted from long bones for RT-PCR analysis. Values are normalized to Hprt. (B) 8-week-old WT and Shn3KD mice were fed PBS- or DOX-containing chow for 6 weeks. Femurs from 14-week-old mice were analyzed by μCT. Shown are bone volume fraction, trabecular number, midshaft bone volume fraction (M.BV/TV), and cortical thickness. (C) 8-week-old WT and Shn3KD mice were fed DOX-containing chow for 6 weeks, and femurs from 14-week-old mice were analyzed by histomorphometric analysis. (D) 18-week-old WT and Shn3KD mice were fed DOX-containing chow for 6 weeks, and femurs from 24-week-old mice were analyzed by μCT. Results are presented as mean + SD. *P < 0.05, **P < 0.005, ***P < 0.0005, Student’s t test.