Fig 3.

Clevudine-TP inhibited protein priming initiated by dCTP and TTP. HP was copurified with the mutant Hε-B6A (coding for priming initiation with TTP; lanes 1 to 4 and 9 to 12) or Hε-B6G (coding for initiation with dCTP; lanes 5 to 8 and 13 to 16). HP bound to Hε-B6A or Hε-B6G was assayed for protein priming as described in the legend to Fig. 2, except [α-³²P]TTP (Hε-B6A) or [α-³²P]dCTP (Hε-B6G) was used instead of [α-³²P]dGTP, in the presence of dH₂O (control; lanes 1, 5, 9, and 13), 100 μM clevudine-TP (CLV-TP; lanes 2 and 6), ddTTP (lanes 3 and 11), ddCTP (lanes 7 and 15), 3TC-TP (lanes 4, 8, 12, and 16), or entecavir-TP (ETV-TP; lanes 10 and 14). Samples were resolved by SDS-PAGE and visualized by autoradiography.