Fig 1.

Effect of PNAs on the production of CmeA, CmeB, and CmeC in C. jejuni. (A) Western immunoblotting showing the inhibition by various PNAs (labeled on top of the panel). C. jejuni NCTC 11168 was treated with the PNAs before the analysis, and the bacterial cells were blotted with specific antibodies against CmeA, CmeB, and CmeC. The CmeC protein is shown as a doublet due to glycosylation. The same amount of total proteins was loaded in each lane, and MOMP was used as an internal control. (B) Densitometric analysis of the immunoblotting results. The CmeA, CmeB, and CmeC levels in the samples treated with the specific PNAs were normalized against the sample treated with the negative-control PNA. Each bar represents the average of two independent immunoblots.