Table 1.

Oligodeoxyribonucleotides used in PCR experiments for diagnosis and cloning purposes^a

Primer	Nucleotide sequence (5′ to 3′)	DNA target	Programb	Amplicon size
V1	AAGTGGTGGTGAAGTAACACG	vga(A)	P1 diagnosis	1 kbp
V2	TCAAGAAAGTTTGTTGGTTCATC
T1	ATATCCGACATCGCCAACTC	Tn5406	P1 diagnosis	740 bp
T2	TTCGTGTTACTTCACCACCAC
X	ATTTCATTATCGCCATCTGTC	EES57021	P2 cloning	2.4 kbp
Y	TCTTCCTTCTTCAATTTCCC
lnuACDf	GGTTWGATGGWGGYTGGGG	lnu(A)	P3 diagnosis	230 bp
lnuAr	AATTGCCACCTTCTGGGTTTGC
lnuBFf	AGARGGTGACSARTWCTCTGA	lnu(B)	P3 diagnosis	400 bp
lnuBFr	AKGMRCGAGCATAYTCTCC

^aThe primer pairs described here were not found in the literature. Other primer pairs were vgaB1 and vgaB2 for detection of vga(B) (24), vgaC-1 and vgaC-2 for detection of vga(C) (21), vgaE_fw and vgaE_rv for detection of vga(E) (22), lsaB-1 and lsaB-2 for detection of lsa(B) (21), cfr_fw and cfr_rv for detection of cfr (23), vatA1 and vatA2 for detection of vat(A) (24), and 16S-27F and 16S-907R for detection of rrs (12).

^bThermocycling programs were as follows: P1, 1 cycle of 5 min at 94°C and 2 min at 54°C; 30 cycles of 60 s at 72°C, 60 s at 94°C, and 60 s at 54°C; and 1 cycle of 10 min at 72°C; P2, 1 cycle of 5 min at 94°C and 2 min at 54°C; 30 cycles of 2 min at 72°C, 1 min at 94°C, and 1 min at 54°C; and 1 cycle of 10 min at 72°C); P3, 1 cycle of 5 min at 94°C and 2 min at 50°C; 30 cycles of 40 s at 72°C, 50 s at 94°C, and 50 s at 50°C; and 1 cycle of 10 min at 72°C.