Fig 7.

(A) Effect of pretreatment with artemisinin monomer (AS), monomer DPP (compound 558), dimer primary alcohol (compound 606), dimer DPP (compound 838), TPP, and GCV on CMV replication. (B) Inhibition of DNA replication, pp28 expression, and virus DNA yield by artemisinin monomer (AS), monomer DPP (compound 558), dimer primary alcohol (compound 606), dimer DPP (compound 838), TPP, and GCV. HFFs were treated with the indicated compounds for 24 h prior to infection. Compounds were removed just before infection. After infection, cells were grown in medium free of compounds. DNA replication in cell lysates collected at 48 hpi was measured by real-time PCR. pp28-luciferase activity was measured in cell lysates collected at 72 hpi. Virus DNA yield in supernatants collected at 96 hpi was measured by real-time PCR. Mean values ± SDs of triplicate determinations from three independent experiments are shown.