Fig 1.

Molecular characterization and antibiogram of isolates showing EMRSA-15 pulsotype. The PFGE dendrogram compares fingerprint patterns of the related isolates from 14 patients. Columns marked “MUP” and “PVL” indicate the results for genetic tests performed to detect the mupA and PVL genes, respectively. CIP, ciprofloxacin; TMSZ, trimethoprim-sulfamethoxazole; GENT, gentamicin; MINO, minocycline; CLIND, clindamycin; N, negative; P, positive; S, susceptible; R, resistant; I, intermediate; †, all isolates belong to spa types included in the cluster ST22, a recognized cluster for EMRSA-15; **, this isolate has a unique pulsotype, although it is closely related to pulsotype A; the spa sequence was unique and not listed in the Ridom SpaServer database; *, this isolate had a distinct PFGE profile and belongs to spa type t005; it is also the only isolate positive for the PVL gene; of note, spa type t005 has been reported to be PVL positive (29).