Fig 5.

41F5 has low toxicity to mammalian cells at concentrations that inhibit Histoplasma yeast growth. Determination of the IC₅₀ of 41F5 for P388D1-lacZ macrophages (A), J774A.1 macrophages (B), or HepG2 hepatocytes (C) was performed. Host cells were cultured with different concentrations of 41F5 or the vehicle-only control (DMSO). After 48 h, the remaining cells were quantified by using crystal violet and normalized to the value for cells cultured in the absence of an inhibitor. Data represent the means ± standard deviations (n = 3). Significant differences determined by Student's t test denote greater toxicity of 41F5 than of the DMSO control, indicated by asterisks (P < 0.05).