Table 3.

RAL susceptibility of viruses containing site-directed Y143X substitutions alone and in combination with secondary substitutions^a

^aRAL susceptibility of pseudoviruses engineered by site-directed mutagenesis containing Y143X substitutions alone or in combination with additional RAL resistance-associated secondary substitutions. Susceptibility was measured using the PhenoSense Integrase assay and expressed as FC in EC₅₀ relative to the wild-type reference virus. RAL FC results are expressed as means of duplicates of each mutant. FC variation between duplicates of each mutant was <1.5. FC values are color coded as follows: light yellow, >2 to <10; yellow, 10 to <20; brown, 20 to <50; red, ≥50.