Table 1.
Oligonucleotides used for amplification and sequencing of domains II and V of the 23S rRNA gene
| 23S rRNA domain and locus | Primer | Sequence (5′–3′) | Reference |
|---|---|---|---|
| Domain V | |||
| External-specific primersa | NG23S1-F | GGCTATGAAGGCGGCGATT | 9 |
| NG23S2-F | TTTCAGATGAGTAATGTACACC | 9 | |
| NG23S3-F | CAATCCGCAAGTCTGCCGA | 9 | |
| NG23S4-F | CTCTCCGATCCCGAACTCG | 9 | |
| Internal common primera | NG23S-R | GAAGATGTGCAAGCATCGGA | 9 |
| Nested PCR | NG23s1905-F | ACGGTCCTAAGGTAGCGA | 9 |
| NG23s2769-R | TCTCATCTTCAGGCGAGTT | 9 | |
| Domain II | |||
| Internal common primera | NG752-R | CAACGACTTACATTCAGTAGC | This study |
| Seminested PCRb | NG752-F | TTCTGATACCTCCAGCACAC | This study |
a
External-specific primers were used for specific amplification of each copy of the 23S rRNA gene (4 copies in N. gonorrhoeae) together with each internal common primer (NG23S-R for amplification of domain V and NG752-R for amplification of domain II). The obtained amplicons were used for the subsequent nested or seminested assay.
b
For the seminested PCR, primers NG752-F and NG752-R were used.