Fig 5.

S0 and S3 inhibit the elevation of autophagy induced by IAV. (A) Effects of S0 and S3 on the conversion of LC3I to LC3II determined by Western blotting. A549 cells were infected or not with IAV (MOI = 0.001) and treated or not with ribavirin, S0, or S3. At 8, 16, or 24 h p.i., the cells were collected and subjected to Western blotting using a human LC3 antibody. The zones and average gray values of each band were detected and quantified with BandScan 5.0 software, the multiplication product of the zone area and the average gray value was calculated, and the results are expressed as the ratio of LC3II to β-actin. (B) S0 and S3 inhibited the dot-like aggregation of EGFP-LC3II. A549 cells were transfected with pEGFP-LC3 plasmid and infected with IAV (MOI = 2.0), and at 8 h p.i., the percentages of cells containing EGFP-LC3 dots compared to cells expressing EGFP were calculated. (f) The concentrations of S0, S3, and ribavirin were 100, 80, and 200 μM, respectively. The data are expressed as means ± SD of three independent experiments. *, P < 0.05, and **, P < 0.01 versus the NC group (virus-only group).