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Western blot analysis of 2696r and wild-type (wt) HCMV pUL97. HFF infected with 2696r or the wild type were harvested 4 days postinfection (MOI, ∼1). Proteins from the cells were extracted and were analyzed via Western blotting using an anti-pUL97 antibody. The locations of pUL97 from the wild-type virus and of truncated pUL97 from 2696r are indicated. An anti-actin antibody was used as a loading control. The positions of molecular weight markers (in thousands) are indicated on the left.
