Fig 3.
Effect of the uppS1 allele on the UppS protein level. Strains containing Pspac(hy)-uppS+-FLAG and Pspac(hy)-uppS1-FLAG at the neutral amyE chromosomal locus were grown in LB medium at 37°C in the absence or presence of 0.1 mM IPTG. At an OD600 of 0.4, cells were harvested for protein extraction. A total of 20 μg of protein from each strain was electrophoresed on a 12% acrylamide gel and transferred to PVDF membrane. The signal intensities were quantified using ImageJ software. A representative data set is shown.