Fig 6.
The compensatory-adaptive response of B. subtilis through σM to a decrease in UppS levels. (A) Microarray transcriptional analysis of strain HB13648 (kan-uppS1) and parental strain HB13647 (kan-uppS+). Black triangles indicate the σM regulon genes whose expression changed at least 1.5-fold. The data shown are mean fluorescence intensities from two independent experiments. Each comparison was performed with dye swaps on each of three biological replicates. (B) Effect of inactivation of sigM, sigX, and sigW on the susceptibility of the HB13648 (kan-uppS1) strain to VAN. The MIC (in μg/ml) for VAN was determined in MH broth by using a Bioscreen C growth analyzer. (C) Effect of sigM inactivation on the susceptibility of the HB13648 (kan-uppS1) strain to fosfomycin and d-cycloserine. Disk diffusion assays were performed on MH agar plates with fosfomycin and d-cycloserine. Three independent experiments were performed for each strain. Error bars indicate standard deviations.