Table 1.
In vitro assays on T. cruzia
| Compound | EC50, μM (SI) |
Intracellular forms, Y |
|||
|---|---|---|---|---|---|
| BT, Y | CT |
||||
| Y | Colombiana | EC50, μM (SI) | EC90, μM | ||
| VNI | 11 ± 5 A (18) | 3.1 ± 0.8 (67) | 3.4 ± 0.5 (59) | 0.9 ± 0.2 C (56) | 38 ± 5 |
| VNI/VNF | 32 ± 15 B (6) | ND | ND | 0.9 ± 0.2 D (170) | 7 ± 5 |
| Bz | 13 ± 2 AB (>77) | ND | ND | 2.8 ± 1.9 CD (>360) | ND |
a
The trypanocidal effect (EC50 values and selectivity index [SI]) were evaluated for bloodstream (BT) (Y strain) and tissue culture-derived (CT) (Y and Colombiana strains) trypomastigotes incubated at 37°C for 24 h and on intracellular forms (Y strain, reduction of infection index) incubated for 48 h at 37°C. SIs were determined at 24 h of incubation for BT and CT (EC50 = 200 μM) and at 48 h of incubation for intracellular forms (EC50 = 50 and 150 μM for VNI and VNI/VNF, respectively) (see Materials and Methods). Values are means and standard deviations. The same letter is placed next to values for which statistical evaluation by ANOVA did not show statistical significance (P > 0.05). ND, not done.