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. Author manuscript; available in PMC: 2014 Sep 7.
Published in final edited form as: Analyst. 2013 Jul 8;138(17):4892–4897. doi: 10.1039/c3an01142a

Figure 4.

Figure 4

Flow cytometry analysis of anti-CD95 induced apoptosis using Annexin V-FITC/PI. Apoptotic cells are located in the lower right quantrant. (a) Control (probe without anti-CD95) and (b) blank (no probe or antibody) showed normal levels of nectrotic and apoptotic cells. However, when receptor mediated apoptosis was initiated, apoptotic cells were observed at 3 hours (c). Given the rapid temporal resolution of our mitochondrial-based assay, it is possible to exclude mitochondrial protein release as a co-mechanism during receptor-mediated apoptosis in Ramos cells.