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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Clin Cancer Res. 2013 Jun 18;19(16):4422–4432. doi: 10.1158/1078-0432.CCR-13-0788

Figure 5. LB100-mediated radiosensitization is partially rescued by CDC25C depletion.

Figure 5

A, MiaPaCa-2 cells were transfected with the indicated siRNA pools. Seventy-two hours post-transfection, cells were treated with LB100 for 2 hours pre- and 24 hours post-IR and harvested for immunoblotting for the indicated proteins. B, MiaPaCa-2 cells were transfected and plated for clonogenic survival as illustrated in Fig. 1B with the addition of LB100 2 hours pre- and 48 hours post-IR. Plots shown are representative of 3 independent experiments. Data in the legend are the mean RER ± SE (n = 3). The mean cytotoxicity of siRNA treatments was 1.0 (mock), 0.9 (si-NS), 0.7 (si-NS+LB100), 0.6 (si-CDC25C), and 0.5 (si-CDC25C+LB100) with less than 15% SE. Statistical significance versus Mock* or si-NS+LB100‡ is indicated.