Figure 5.

Cardiac tissues engineered from human induced pluripotent stem (iPS) cell-derived cardiomyocytes. (A): The polyacrylic tissue engineering chamber placed around the femoral artery and vein in the groin region of a nude rat. A plasma clot containing human iPS cells (arrow) was implanted at the base of the chamber and folded over the femoral vessels (B). (C): Engineered tissue construct at 4 weeks postimplantation. (D–G): Cardiac tissue constructs were stained with cardiac troponin T (D, E), α-actinin (F), and myosin heavy chain (G). (H, I): Serial sections stained with cTnT (H) and connexin 43 (I). Magnification, ×400 (D, E, H) and ×200 (F, G). Scale bars = 5 mm (A–C) and 50 μm (D–I). Abbreviations: a, femoral artery; cTnT, cardiac troponin T; v, femoral vein.