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. 2013 Sep 15;140(18):3731–3742. doi: 10.1242/dev.096560

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© 2013. Published by The Company of Biologists Ltd

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Fig. 2. — Dkk1 overexpression abrogates initial lung formation. (A-D) β-Gal staining on E10.5 control (ctrl) TOPGAL (A), Rosa26-rtTA;Tet-Dkk1^+/-;TOPGAL (B), Rosa26-rtTA;Tet-Dkk1^+/+;TOPGAL (C) and Rosa26-rtTA;Tet-Dkk1^+/+;Tet-Fgf10;TOPGAL (D) lungs/foreguts dox-induced at E8.0. (E-H) Nkx2.1 immunostaining on E10.5 ctrl (E), Rosa26-rtTA;Tet-Dkk1^+/- (F), Rosa26-rtTA;Tet-Dkk1^+/+ (G) and Rosa26-rtTA;Tet-Dkk1^+/+;Tet-Fgf10 (H) lungs/foreguts dox-induced at E8.0. (I-L) Foxa2 immunostaining on E10.5 ctrl (I), Rosa26-rtTA;Tet-Dkk1^+/- (J), Rosa26-rtTA;Tet-Dkk1^+/+ (K) and Rosa26-rtTA;Tet-Dkk1^+/+;Tet-Fgf10 (L) lungs/foreguts dox-induced at E8.0. (M,N) qPCR analysis of relative Dkk1 (M) and Fgf10 (N) mRNA abundance on E10.5 Rosa26-rtTA;Tet-Dkk1^+/+ and Rosa26-rtTA;Tet-Dkk1^+/+;Tet-Fgf10 embryos dox-induced at E8.0. ^**P<0.01 (Student’s t-test); n≥3. Error bars represent standard error. (O,P) Whole-mount Fgf10 in situ hybridization on an E10.5 Rosa26-rtTA;Tet-Dkk1^+/+ embryo (O) and a Rosa26-rtTA;Tet-Dkk1^+/+;Tet-Fgf10 embryo dox-induced from E10.5 onwards (P). n≥3. Scale bars: 50 μm (E-L).