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. 2013 Sep 15;140(18):3731–3742. doi: 10.1242/dev.096560

Fig. 8.

Fig. 8.

Fgf(10) signaling regulates basal cell differentiation in the trachea. (A-D) Immunostaining for p63 and K5 on E18.5 wild-type (A), Fgf10-/- (B), Rosa26-rtTA;Tet-sFgfr2b induced from E15.5 (C) and E15.5 wild-type (D) tracheas. Insets show high magnification images of boxed areas. (E) qPCR analysis of relative Scgb1a1, FoxJ1, Cgrp, keratin 5 and p63 mRNA abundance on E18.5 lungs from wild-type mice, Rosa26-rtTa;Tet-Dkk1 mice induced from E10.5 and Rosa26-rtTA-Tet-Fgf10 mice induced from E15.5. (F) qPCR analysis of relative keratin 5 and p63 mRNA abundance on E18.5 tracheas from wild-type mice, Fgf10-/- mice and Rosa26-rtTA-Tet-sFgfr2b mice induced from E15.5. **P<0.01, *P<0.05 (Student’s t-test). n≥3. (To allow for better visualization Scgb1a1, Foxj1 and Cgrp expression levels presented in the graph are reduced by a factor of 1500, 10 and 5, respectively.) Error bars represent standard error. Scale bars: 100 μm (A-D); 25 μm (insets in A-D).