Table 4.
Intracellular staining for IL-17a, IL-4, IFN-γ, and TNF-α for allogeneic T lymphocytes and naive CD4+ T lymphocytes cocultured with MoDCs previously exposed to LPS, E. amstelodami, W. sebi, S. rectivirgula, or M. immunogenum
| Cells tested | % (mean ± SD) |
CD4+ CD25+ FoxP3+ cellsa (% [mean ± SD] of parents) | |||
|---|---|---|---|---|---|
| IL-17a | IFN-γ | IL-4 | TNF-α | ||
| T lymphocytes cocultured with MoDCs exposed to microbial extracts | |||||
| Naive CD4+ T lymphocytes | 0.1 ± 0.08 | 1.2 ± 0.7 | 0.7 ± 1.2 | 3 ± 0.5 | NR |
| Naive CD4+ T lymphocytes + LPS-exposed MoDCs | 0.17 ± 0.1 | 13.5 ± 5 | 1.1 ± 1.5 | 7.7 ± 0.7 | NR |
| Naive CD4+ T lymphocytes + EA-MoDCs | 0.1 ± 0 | 6.8 ± 1.9 | 1.5 ± 2 | 6.6 ± 0.9 | NR |
| Naive CD4+ T lymphocytes + WS-MoDCs | 0.13 ± 0.06 | 7.9 ± 4.9 | 0.7 ± 0.6 | 5 ± 0.4 | NR |
| Naive CD4+ T lymphocytes + SR-MoDCs | 0.1 ± 0 | 6.5 ± 1.1 | 0.9 ± 5 | 7.9 ± 1.3 | NR |
| Naive CD4+ T lymphocytes + MI-MoDCs | 0.1 ± 0 | 8.9 ± 0.4 | 1.5 ± 3 | 4.3 ± 0.4 | NR |
| Polarization controls | |||||
| Naive CD4+ T lymphocytes, Th1 | 0.1 ± 0 | 60.8 ± 1.5 | 21.2 ± 2.3 | 18.4 ± 1.6 | NR |
| Naive CD4+ T lymphocytes, Th2 | 0.1 ± 0 | 0.1 ± 0 | 19.2 ± 0.8 | 0.9 ± 0.3 | NR |
| Naive CD4+ T lymphocytes, Th17 | 25 ± 7.5 | 0.3 ± 0.1 | 0 ± 0 | 0 ± 0 | NR |
| Naive CD4+ T lymphocytes, Treg | 0 ± 0 | 0.4 ± 0.08 | 0 ± 0 | 0 ± 0 | 55 ± 9.8 |
a
Specific CD25/FoxP3 staining was added for the Treg polarization control. NR, no response.