Table 5.
Cytokine/chemokine levels in supernatants of allogeneic T lymphocytes, naive CD4+ T cells cocultured with MoDCs previously exposed to LPS, S. rectivirgula, M. immunogenum, W. sebi, or E. amstelodami, and T cell polarization controlsa
| Cells tested | Cytokine/chemokine levels (pg/ml [mean ± SD]) in: |
||||||
|---|---|---|---|---|---|---|---|
| IL-8 | IL-6 | IFN-γ | TNF-α | IL-10 | IL-17a | IL-4 | |
| T lymphocytes cocultured with MoDCs exposed to microbial extracts | |||||||
| Naive CD4+ T lymphocytes | 34 ± 7 | 0 ± 0 | 19 ± 4.4 | 7 ± 4.5 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Naive CD4+ T lymphocytes + LPS-exposed MoDCs | 3,888 ± 277 | 2,005 ± 577 | 98,266 ± 4,520 | 2,343 ± 423 | 27 ± 5 | 9 ± 4 | 0 ± 0 |
| Naive CD4+ T lymphocytes + EA-MoDCs | 4,172 ± 550 | 1,983 ± 346 | 67,676 ± 2,732 | 3,529 ± 637 | 156 ± 39 | 31 ± 12 | 0 ± 0 |
| Naive CD4+ T lymphocytes + WS-MoDCs | 4,169 ± 139 | 2,223 ± 737 | 56,123 ± 2,767 | 3,451 ± 470 | 46 ± 18 | 16 ± 3 | 0 ± 0 |
| Naive CD4+ T lymphocytes + SR-MoDCs | 3,861 ± 315 | 1,673 ± 305 | 51,254 ± 2,223 | 1,769 ± 310 | 29 ± 10 | 12 ± 4 | 0 ± 0 |
| Naive CD4+ T lymphocytes + MI-MoDCs | 4,041 ± 190 | 2,279 ± 508 | 56,704 ± 2,895 | 2,703 ± 563 | 33 ± 12 | 15 ± 6 | 0 ± 0 |
| Polarization controls | |||||||
| Naive CD4+ T lymphocytes, Th1 | 6,058 ± 358 | 2,247 ± 252 | 4,714 ± 547 | 2,813 ± 926 | 393 ± 170 | 47 ± 11 | 0 ± 0 |
| Naive CD4+ T lymphocytes, Th2 | 44 ± 16 | 276 ± 56 | 116 ± 46 | 946 ± 329 | 1,320 ± 344 | 42 ± 16 | 36,957 ± 6,276 |
| Naive CD4+ T lymphocytes, Th17 | 443 ± 130 | 1,743 ± 712 | 177 ± 86 | 1,082 ± 435 | 1,500 ± 522 | 511 ± 218 | 0 ± 0 |
| Naive CD4+ T lymphocytes, Treg | 1,620 ± 780 | 852 ± 290 | 62 ± 17 | 2,081 ± 637 | 223 ± 78 | 42 ± 18 | 0 ± 0 |
a
Cytokine/chemokine levels were measured in supernatants using the Luminex technique after 6 days of incubation.