Table 6.

Comparison of results obtained in phenotypic and functional assays using different batches of S. rectivirgula and E. amstelodami total extracts, either below or above the 0.01 EU/ml limit

Polarization result	Extracts with >0.01 EU/mla		Extracts with <0.01 EU/mla		Differenceb
	SR- MoDCs	EA-MoDCs	SR- MoDCs	EA-MoDCs	SR- MoDCs	EA-MoDCs
Phenotypic assays
% with CD80	51 ± 19	60 ± 18	61.2 ± 15.2	58.3 ± 15.2	NS	NS
% with CD86	45 ± 18	66 ± 16	38.8 ± 19	58.4 ± 13.1	NS	NS
IL-23 level (pg/ml)	72 ± 38	2,416 ± 875	122 ± 46	2,590 ± 422	NS	NS
IL-8 level (pg/ml)	1,987 ± 358	2,708 ± 296	3,876 ± 823	2,189 ± 752	0.03	NS
IL-10 level (pg/ml)	79 ± 52	475 ± 89	86 ± 48	546 ± 66	NS	NS
Functional assays
% with CD4 (CFSE)	48 ± 19.6	36.4 ± 11.2	73 ± 27	30.2 ± 7.9	NS	NS
T-bet mRNA expression (fold)	4.16 ± 1.8	2.9 ± 1.2	4.78 ± 1.3	3.3 ± 1.6	NS	NS
% with intracellular TNF-α	7.6 ± 0.2	7 ± 0.5	8.2 ± 2.4	6.1 ± 0.9	NS	NS
% with intracellular IFN-γ	6.1 ± 0.2	8 ± 2.3	6.8 ± 1.8	5.6 ± 1.6	NS	NS
Il-8 level (pg/ml)	3,522 ± 243	4,356 ± 487	4,200 ± 386	3,987 ± 612	NS	NS
Il-6 level (pg/ml)	1,423 ± 253	2,289 ± 423	1,923 ± 358	1,678 ± 269	NS	NS
IFN-γ level (pg/ml)	53,620 ± 1,245	75,283 ± 2,641	48,888 ± 3,200	60,069 ± 2,823	NS	0.044

^aValues are means ± SD.

^bDifferences were tested using the Wilcoxon signed-rank test. NS, not significant.