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Expression and purification of rOmp31 antigen in the pQE30UA vector. Lane 1, uninduced; lane 2, induced; lane 3, pellet; lane 4, lysate; lane 5, flowthrough; lane 6, wash; lane 7, prestained protein marker (Fermentas no. SM0671); lane 8, eluate 1; lane 9, eluate 2; lane 10, eluate 3. Molecular mass markers are shown on the right.
