Fig 2.

Kinetics of protein-specific serum antibodies directed against VP2 of BTV-8, VP7 of BTV, and NS1 and NS2 of BTV-2 in vaccinated cows. Animals (represented by group means, n = 5) were vaccinated twice, with a 3-week interval, with an experimental subunit vaccine (SubV), a commercial inactivated vaccine (CV), or PBS (control) (arrows). Sera were collected at weeks 0, 3, 6, and 9 and tested by competitive ELISA for BTV-8 VP2-specific antibodies (a), indirect ELISA for BTV-2 NS1-specific IgG1 antibodies (b), indirect ELISA for BTV-2 NS2-specific IgG1 antibodies (c), and double-antigen sandwich ELISA for BTV VP7-specific IgM and IgG antibodies (d). Corrected OD (COD) values are presented as percent inhibition (a), percentage of positive reference serum values (b and c), or 100% minus competition percentage (d). Vertical lines, standard deviations. *, P ≤ 0.05, and **, P ≤ 0.01, for comparisons with the indicated groups.