Fig 5.

Serum bactericidal antibody responses of transgenic mice immunized with mutant recombinant fHbp vaccines that do not bind fH. C57BL/6 mice that lacked endogenous mouse fH and expressed a chimeric mouse-human fH molecule that permitted binding to fHbp were immunized with three doses of a wild-type (WT) fHbp vaccine that bound fH or three mutant vaccines with decreased fH binding (E218A/E239A double mutant, R41A, or I246A). The serum bactericidal GMT of mice given the WT fHbp vaccine was 1:22, which was lower than that for each of the three mutant fHbp vaccines tested (1:55, 1:32, and 1:72); none of the pairwise comparisons with the WT vaccine were significant (P > 0.05). Geometric mean titers (GMT) are shown as horizontal bars as calculated from data in Fig. 5B of the original paper (48). The amino acid residues have been renumbered based on the mature fHbp ID 1 protein sequence (http://pubmlst.org/neisseria/fHbp). (Modified from reference 48 with permission of the publisher.)