Table 3.
Antigen/mitogen-induced proliferation of spleen cells from mice immunized with native BCG and recombinant BCG-pDE22-ESAT6
| Spleen cell stimulanta | No. of mice positive/no. tested, by immunization typeb |
||
|---|---|---|---|
| Native BCG | rBCG-pDE22-ESAT6 | rBCG-pDE22-ESAT6 (boosted) | |
| ConA | 5/5 | 2/2 | 3/3 |
| BCG sonicate | 5/5 | 2/2 | 3/3 |
| MT-CF | 5/5 | 2/2 | 3/3 |
| ESAT6 peptide pool | 0/5 | 0/2 | 0/3 |
| P1 | 0/5 | 0/2 | 0/3 |
| P2 | 0/5 | 0/2 | 0/3 |
| P3 | 0/5 | 0/2 | 0/3 |
| P4 | 0/5 | 0/2 | 0/3 |
| P5 | 0/5 | 0/2 | 0/3 |
| P6 | 0/5 | 0/2 | 0/3 |
a
ConA, concanavalin A; MT-CF, M. tuberculosis culture filtrate.
b
The proliferation response was considered positive with a stimulation index (SI) of ≥2.