Fig 3.

Expression of CD40 on PBMCs isolated from healthy control cows and cows naturally infected with M. avium subsp. paratuberculosis in the subclinical and clinical stages of infection. Unfractionated PBMCs were cultured in complete medium for 7 days and then were incubated for an additional 8, 24, and 72 h in complete medium (A) or complete medium with live M. avium subsp. paratuberculosis (B) at a 10:1 MOI. Data are presented as percentage of cells (mean ± SEM). Significant differences between treatment groups within a time point are designated by different letters (P < 0.05). Significant differences due to the time of incubation are discussed in the text.