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Microneutralization assay. The titers of antibodies against recombinant NL/1/00 and recombinant NL/1/99 after immunization with M2, WT, or DMEM are shown. Sera were collected at 5, 14, 28, and 56 days p.i. (dpi). All serum samples were tested in the microneutralization assay against NL/1/00 or against NL/1/99. Eight mice were in each group. P < 0.001 for the M2 and WT groups versus the DMEM group (one-way ANOVA, two-tailed). Dotted line, limit of detection; horizontal bars, means.
