Table 4.
Number of copies of virus RNA in mouse lungs 5 days after challenge with recombinant NL/1/99a
| Time of challenge (day p.i.) | Inoculum | Mean log10 no. of copies/g ± SD) | Reduction of mean log10 no. of copies/g |
|---|---|---|---|
| 28 | DMEM | 8.18 ± 0.43 | |
| 28 | M2 | 4.66 ± 0.83 | 3.52 |
| 28 | WT | 4.31 ± 0.82 | 3.87 |
| 56 | DMEM | 8.23 ± 0.49 | |
| 56 | M2 | 4.70 ± 0.79 | 3.53 |
| 56 | WT | 4.79 ± 0.56 | 3.44 |
a
All of the mice inoculated with the WT or M2 virus or mock inoculated were challenged with recombinant hMPV strain NL/1/99 at 28 or 56 days after the initial inoculation. Five days later, mouse lungs were harvested for viral RNA measurement by real-time PCR. At 28 days p.i., P < 0.001 for the M2 and WT groups versus the DMEM group; at 28 days p.i., P = 0.34 for the WT group versus the M2 group. At 56 days p.i., P < 0.001 for the M2 and WT groups versus the DMEM group; at 56 days p.i., P = 0.77 for the WT group versus the M2 group. All P values were determined by one-way ANOVA.