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Detection of influenza virus NP on the surface of A549 cells infected with various influenza A and B viruses. Cells were infected with various influenza viruses at an MOI of 3 and incubated for 16 to 18 h. Subsequently, cells were incubated with MoAb NP (white) or isotype control antibody (gray; 20 μg/ml), and binding of these antibodies was assessed after subsequent incubation with an FITC-labeled MoAb directed against human IgG by flow cytometry.
