Fig 1.
Hac1 enhances Gcn4 activity during amino acid starvation. The diploid gcn4 deletion strain (RH2694) expressing wild-type GCN4 (pME1092), the substituted GCN4L267S (pME2901) or GCN4L253G (pME3378) from CEN plasmids with GCN4 promoter and terminator, or, as a control, the empty vector (CEN) (pRS314) was additionally transformed with either independently overexpressed HAC1 (MET25-HAC1) (pME3498) or an empty vector (p426MET25). Strains were spotted in 10-fold dilutions either on YNB as a control or on YNB medium containing 5 mM 3AT to induce amino acid starvation. After incubation for 3 to 4 days at 30°C, plates were photographed.