Fig 6.

Effect of Hac1 and Gcn4 on FLO11::lacZ promoter elements. The β-galactosidase activity of 14 isolated 400-bp elements of the FLO11 promoter region was assayed in diploid wild-type cells (RH2656) or in corresponding cells deleted for the transcription factor HAC1 (RH3412) or GCN4 (RH2658) (see Table S1 in the supplemental material). The activity was determined under nonstarvation conditions (YNB, upper graphs) or in the presence of amino acid starvation (3AT, lower graphs) induced by the addition of 10 mM 3AT. Each breakpoint represents the measured reporter construct expression less the measured expression of the construct without insert (pME2212) in the indicated genetic background. β-Galactosidase activities measured in the wild type are shown by diamonds, Δhac1/Δhac1 breakpoints are shown by squares, and Δgcn4/Δgcn4 breakpoints are shown by triangles.