Table 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| B3782 | 3-kbp FLO11::lacZ in YEp355 | 53 |
| p180 | GCN4::lacZ reporter construct (uORFs 1 to 4) on a centromere vector (URA3) | 59 |
| p227 | GCN4::lacZ reporter construct (without uORFs) on a centromere vector (URA3) | 73 |
| pAG25 | NatMX4 cassette in pFA6 | 74 |
| p426MET25 | pRS426 containing MET25 promoter, CYC1 terminator | 75 |
| pFLO11-2/1 to pFLO11-15/14 | 440-bp sequence elements cloned into pLG669Z from bp −1 to −420, bp −180 to −620, and bp −380 to −1020 until bp −2580 to −2980 | 53 |
| pLG669Z | lacZ shuttle vector | 76 |
| pMCZ-Y | UPRE-CYC1-lacZ reporter construct on multicopy vector (URA) | 23 |
| pME1092 | 2.8-kb GCN4 fragment in pRS314 | 57 |
| pME1112 | Integrative GCRE6::lacZ reporter construct | 57 |
| pME2212 | pLG669Z without UASa | 18 |
| pME2213 | Integrative FLO11::lacZ reporter construct | Our collection |
| pME2901 | GCN4prom-GCN4L267S-GCN4-term in pRS314 | 17 |
| pME3378 | GCN4prom-GCN4L253G-GCN4-term in pRS314 | 17 |
| pME3498 | MET25prom-HAC1 in p426MET25 | This study |
| pRS314 | TRP CEN Ampr ori | 77 |
| pRS426 | URA3 2μm Ampr ori | 77 |
a
UAS, upstream activation sequence.