Fig 2.

hstD/Aohst4 and hdaD/Aohos2 regulate SM production and development. (A) The morphological phenotype on N agar medium (MM) and results of the kojic acid production plate assay (KA) are provided for the indicated strains. (B, C) Radial growth and conidiation of the indicated strains on N agar medium, respectively. (D) Time course characteristics of kojic acid production of the indicated disruptants. (E) Expression profiles of kojic acid cluster genes represented by Northern hybridization. The culture times of the indicated strains are shown at the top of the panel. The analyzed gene is indicated on the left side of each blot. The results for rRNA, used as the loading control, are shown. (F) Bioassay of penicillin production of the ΔhstD strain. (G) Northern hybridization of the penicillin biosynthetic gene ipnA in the ΔhstD strain. The results for rRNA, used as the loading control, are shown. The adeA⁺ strain was used as a control in the experiment whose results are presented in this figure. All data are represented as means ± SDs (n = 3); *, P < 0.01, t test.