Fig 3.

Total ion chromatograms of acidic metabolites detected in 22-h-old cultures of strain B356 grown on diphenylmethane (black line) or in resting cell suspensions of E. coli carrying plasmids pQE31[B356-bphAE] and pYH31[LB400-bphFGBC] incubated for 30 min with diphenylmethane (gray line) (A) or acidic metabolites produced by a suspension of biphenyl-induced cells of strain B356 incubated for 60 min with benzophenone (B). The protocols are described in Materials and Methods. Shown are the peaks of the TMS-treated metabolites that were identified from their mass spectral features. The unlabeled peaks were also detected in controls unexposed to the substrate. OTMS, trimethylsilyl-oxygen complex.