Fig 4.
Total ion chromatograms of metabolites produced from diphenylmethane by purified preparations of BphAEB356 (black line) and BphAELB400 (gray line) (A) or by a coupled reaction composed of purified BphAEB356 plus BphBB356 (B). (C) Total ion chromatograms of metabolites produced from benzophenone by purified preparations of BphAEB356 (light gray line), BphAELB400 (black line), and BphAEp4 (dark gray line). (D) Total ion chromatograms of metabolites produced from benzophenone by a coupled reaction composed of purified BphAEB356 plus BphBB356. The procedures to purify the enzymes, to set up the reactions, and to extract the metabolites for GC-MS analysis are described in Materials and Methods. Shown are the peaks of nBuB- or TMS-treated metabolites that were identified from their mass spectral features. The unlabeled peaks were also present in controls unexposed to the substrate.