Table 2.
Plasmids and DNA oligomers used in this study
| Plasmid or DNA oligomer | Relevant features or sequence (5′–3′) | Reference |
|---|---|---|
| Plasmids | ||
| pCATam1 | Renamed from pRSVCATam1.2.5; pBR322 derivative harboring a CAT reporter gene with UAG as an initiation codon | 24 |
| pCATam1metYCUA | Renamed from pRSVCATam1.2.5trnfMU35A36; pBR322 derivative harboring a CAT reporter gene with UAG as an initiation codon and expressing tRNAfMet with a CUA anticodon | 24 |
| pCATam1metY | Renamed from pRSVCATam1.2.5trnfM; a pBR322 derivative harboring a CAT reporter gene with UAG as an initiation codon and expressing tRNAfMet | 24 |
| pSG25-lacZ | Construct with wild-type lacZ | 16 |
| pSG-lac7(+1) | Production of functional LacZ from this construct requires +1 frameshifting in the frameshift window | 16 |
| pSG12DP(−1) | Production of functional LacZ from this construct requires −1 frameshifting in the frameshift window | 16 |
| pSGlac10(−1) | Production of functional LacZ from this construct requires −1 frameshift in the frameshift window; the frameshift occurs at a codon different from pSG12DP | 15 |
| pCP20 | Ampr and Cmr plasmid that shows tempurature-sensitive replication and thermal induction of flippage recombinase (Flp) synthesisa | 25 |
| DNA oligomers | ||
| rsmDfp | TTCATGGCACAGCGTTAACG | 8 |
| rsmDrp | AACGAAGATATTCAGCGGGC | 8 |
| rsmBfp | CTCTCGTCGGGAATGGTTTG | 8 |
| rsmBrp | CTGCTCTGGTGCGATCAGAT | 8 |
| S9del3fp | GTCGGTCTGCGTAAAGCACGTCGTCGTCCGCAGTTCTAGTAAGCTTGTGTAGGCTGGAGCTGCTTCG | 8 |
| S9del3rp | TTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAACATATGAATATCCTCCTTA | 8 |
a
Cmr, chloramphenicol resistance; Ampr, ampicillin resistance.