Table 3.
Fold changes in β-galactosidase activity at 37°C
| Frameshift construct | Mean fold change in β-galactosidase activity ± SD at 37°Ca |
||||||
|---|---|---|---|---|---|---|---|
| C | ΔrsmB | ΔrsmD | S9Δ3 | S9Δ3ΔrsmD | S9Δ3ΔrsmB | S9Δ3ΔrsmBΔrsmD | |
| pSG25 (WT) | 1 | 0.73 ± 0.06 | 0.92 ± 0.023 | 1 ± 0.09 | 1 ± 0.03 | 0.84 ± 0.07 | 0.89 ± 0.05 |
| pSG-lac7(+1) | 1 | 1.04 ± 0.1 | 1.29 ± 0.14 | 2.7 ± 0.26 | 2.6 ± 0.26 | 2.9 ± 0.13 | 2.35 ± 0.08 |
| pSG12DP(−1) | 1 | 1.13 ± 0.06 | 1 ± 0.05 | 2.71 ± 0.29 | 2.9 ± 0.14 | 4.13 ± 0.29 | 3.73 ± 0.23 |
| pSGlac10(−1) | 1 | 0.9 ± 0.03 | 1.43 ± 0.1 | 1.8 ± 0.13 | 2.2 ± 0.2 | 6.7 ± 1.8 | 4 ± 0.47 |
a
Fold differences in β-galactosidase activities were calculated from pooled data (from independent experiments) for each of the plasmid constructs in the various strains, and values shown are relative to that for E. coli BW (column C, taken as 1).