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G3P and DHAP transport kinetics in purified R. prowazekii. Kinetic analysis of [14C]G3P (A) and [32P]DHAP (B) transport was performed with purified R. prowazekii suspended in modified SPGMg2+ buffer and assayed at 34°C over the indicated substrate concentration range. The initial linear transport rate was determined by filtration and washing. Hanes-Woolf kinetic plots are shown. Error bars represent standard deviations of the mean of at least three independent rickettsial preparations.
