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The CodY box is required for binding of CodY to the ilvE promoter in S. mutans. CodY binding assays for full-length and truncated (NCB) ilvE promoter regions (described in Materials and Methods) were performed with isoleucine (10 mM) and purified recombinant CodY (1,500 nM). Lanes 1 and 2, ilvE promoter; lanes 3 and 4, truncated ilvE promoter (NCB).
