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The ilvE mutant displays decreased transcriptional activity from the ilvE promoter. CAT activities are shown for ilvE promoter-cat fusions in the UA159 parent strain and ΔilvE backgrounds, grown in BHI medium. Transcriptional activity was determined for three independent cultures in duplicate for each growth condition, as described in Materials and Methods. Statistical significance was determined by Student's t test. *, P ≤ 0.00001 (n = 3). n.s., not statistically significant.
