Table 2.
Oligonucleotide primers used in this study
| Primer application and name | Nucleotide sequence (5′–3′)a | Restriction site |
|---|---|---|
| Primers for cloning | ||
| 1662P8DN (codY) | ATTGATGATTTTCTTGACTTAGGG | |
| 1662P7UP (codY) | ATCACAGCGGTAAAACCTCAGGCG | |
| 1442P8DN (ccpA) | AACCAAGCGGTACCATAGATAC | |
| 1442P7UP (ccpA) | AAATGGGCTATTGCTAGTGATG | |
| 1662BglUpb | CATAGATCTAAGGGCTTTCATGAAAATTGTCGA | BglII |
| 1662BglDownb | CATAGATCTGGTATAATCAATTGAAATCAGTGCTTTACTCATT | BglII |
| Primers for EMSAs and CAT constructs | ||
| 1099Fwdc | CATGAGCTCGATAACATTTCTTACAAAAAGACAGG | SacI |
| 1099Revc | CATGGATCCTTTAATACCTCTTTCCTCG | BamHI |
| 1099RevNCBc,d | CATGGATCCTTTAAAAGATAAAATGGAGCAG | BamHI |
| BglIIATPe | ATTCAACATAGATCTTTCTCCTTC | BglII |
| BRLSMp567>e | ACCTAATATTTATAAGATAAG | |
| Primers for protein expression | ||
| CodYFwd | GAAAGCCCATATGGCTAATTTATTATCG | NdeI |
| CodYRev | GAGTGACTTGGATCCTTAACTATAGTC | BamHI |
a
Restriction sites are underlined.
b
Primer used for cloning of codY complement.
c
Primer used for ilvE target.
d
Primer used to construct a truncated ilvE promoter without the CodY consensus binding sequence.
e
Primer used for ATPase control probe SMU.1534c (3).