Fig 1.

Location of mutations in NodD1. (A) Domain structure of NodD1 containing an N-terminal wHTH DBD (residues 23 to 42), LH (residues 59 to 87), RD I (residues 88 to 165 and 271 to 308), and RD II (residues 171 to 265). Domain borders are approximate and are based on domains in known LTTRs (40, 42, 57, 61). (B) β-Galactosidase activity (Miller units) of a nodC′-′lacZ reporter in S. meliloti A2105 expressing the indicated NodD1 wild-type or point mutation in the absence (white) or presence (black) of 3 μM luteolin. Data are from at least four independent assays except for mutations R65C, K205A, and D284A, for which the data are from two independent assays.