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nodC′-′lacZ activity of S. meliloti expressing wild-type NodD1, NodD1 L69F, NodD1 S104L, NodD1 D134N, and NodD1 M193I (A) or wild-type NodD1, NodD1 K205N, NodD1 L280F, and NodD1 D284N (B) in response to 0 to 20 μM luteolin. Data are plotted as the means ± SD and represent ≥4 independent assays except for NodD1 S104L (n = 2). S. meliloti-expressing vector alone gave <16.5 Miller units of β-galactosidase activity under all conditions.
