Fig 2.
ropA1, but not ropA2, is recalcitrant to genetic disruption. Ten locations targeted for single-crossover disruption are marked by vertical dashed lines. Colony yields for the attempted disruptions are shown by vertical bars (n = 9; error bars represent the standard errors of the means [SEM]). Four colonies from each of the 10 attempts were subsequently tested by PCR for the presence of the desired disruption. Negative results from this test indicate off-target integration elsewhere in the genome. The location of the tRNASer gene is indicated with an asterisk.