Table 1.
Strains, plasmids, and bacteriophages used in this study
| Strain, plasmid, or bacteriophage | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | E. coli cloning strain | 43 |
| B001 | DH5α harboring helper plasmid pRK600 | 44 |
| Rm1021 | S. meliloti SU47 Smr (progenitor to strains listed below) | 45 |
| B199 | lpsB::Tn5-110 Smr, Nmr | 8 |
| B912 | Rm1021 ropA1G129D N3r | This study |
| B920 | Rm1021 ropA1G84D ΦM12r | This study |
| B955 | Rm1021 ropA1G84A ΦM12r | This study |
| B956 | Rm1021 ropA1G84V ΦM12r | This study |
| B957 | Rm1021 ropA1G84R ΦM12r | This study |
| B958 | Rm1021 ropA1ΔA122-N124 ΦM12r N3r | This study |
| B959 | Rm1021 ropA1ΔG203-V204 ΦM12r N3r | This study |
| B961 | Rm1021 ropA1S87Y ΦM12r N3r | This study |
| B962 | Rm1021 ropA1S87F ΦM12r N3r | This study |
| B970 | Rm1021 ropA1205::GV ΦM12r N3r | This study |
| B971 | Rm1021 ropA1D134Y N3r | This study |
| B972 | Rm1021 ropA1ΔN124-D125 N3r | This study |
| B973 | Rm1021 ropA1126::ND N3r | This study |
| B974 | Rm1021 ropA1A199V ΦM12r N3r | This study |
| C540 | Rm1021 ropA1S89P ΦM12r N3r | This study |
| C551 | Rm1021 ropA1ΔV204-T205 resistant to other phages (see Table 3) | This study |
| C566 | Rm1021 ropA1ΔN121-D123 ΦM12r N3r | This study |
| C617 | ropA2::pJG584 | This study |
| Plasmids | ||
| pRF771 | Empty vector for Ptrp transcriptional fusions; Tcr | 46 |
| pRK600 | Self-transmissible helper plasmid; Cmr | 47 |
| pRK7813 | RK2 derivative carrying pUC9 polylinker and λ cos site; Tcr | 16 |
| pJG110 | Transposon delivery vector; Km/Nmr, Apr | 8 |
| pJG194 | 2.2-kb mobilizable suicide vector; Km/Nmr | 8 |
| pJG396 | Wild-type ropA1 (entire coding region) cloned into pRK771; Tcr | This study |
| pJG581 | A 367-bp internal fragment of ropA1 cloned into pJG194 | This study |
| pJG582 | A 334-bp internal fragment of hisC4 cloned into pJG194 | This study |
| pJG583 | A 405-bp fragment upstream of ropA1 cloned into pJG194 | This study |
| pJG584 | A 314-bp internal fragment of ropA2 cloned into pJG194 | This study |
| pJG624 | A 320-bp internal fragment of hisC4 cloned into pJG194 | This study |
| pJG627 | A 330-bp fragment upstream of ropA1 cloned into pJG194 | This study |
| pJG628 | A 319-bp internal fragment of ropA1 cloned into pJG194 | This study |
| pJG629 | A 291-bp internal fragment of ropA1 cloned into pJG194 | This study |
| pJG630 | A 333-bp internal fragment of SMc02397 cloned into pJG194 | This study |
| pJG631 | A 330-bp internal fragment of ropA2 cloned into pJG194 | This study |
| Bacteriophages | ||
| ΦM1 | S. meliloti lytic phage | 6 |
| ΦM5 | S. meliloti lytic phage | 6 |
| ΦM6 | S. meliloti lytic phage | 6 |
| ΦM7 | S. meliloti lytic phage isolated from an alfalfa field | 6 |
| ΦM9 | S. meliloti lytic phage isolated from a commercial inoculant | 6 |
| ΦM10 | S. meliloti lytic phage isolated from a commercial inoculant | 6 |
| ΦM12 | S. meliloti lytic phage isolated from a commercial inoculant | 6 |
| ΦM14 | S. meliloti lytic phage isolated from a commercial inoculant | 6 |
| ΦM19 | S. meliloti lytic phage | 6 |
| N3 | S. meliloti lytic phage isolated from an alfalfa field | 7 |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Nmr, neomycin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance.