Table 2.
Name | Sequencea | Direction | Purpose |
---|---|---|---|
oJG664 | CAGTTTACTTTGCAGGGCTTCC | Forward | Sequence verification of pJG194 inserts |
oJG1243 | TGCGAAAAAGGATGGATATACCG | Reverse | Sequence verification of pJG194 inserts |
oJG524 | GGTGGCGCACTTCCTGATAGC | Forward | Sequence verification of pRF771 inserts |
oJG525 | CGTTATCAGAACCGCCCAGACC | Reverse | Sequence verification of pRF771 inserts |
oMC023 | CGCTCTAGACCCAGACCCGTTTGAAACTTTTG | Forward | Clone ropA1 into pRF771 |
oMC024 | CGCGGATCCGTAGCCATACTCCAGAAAAGAG | Reverse | Clone ropA1 into pRF771 |
oMC029 | CGAAAGCCTACGATCACAGG | Forward | Sequencing of ropA1 mutants |
oMC030 | CGAAGAAGAGGTGCTGTTCC | Reverse | Sequencing of ropA1 mutants |
oMC303 | CGCGGATCCTGAAGCCTACATCCAGCTCG | Forward | Clone a 367-bp fragment of ropA1 into pJG194 |
oMC304 | CGCTCTAGAGTAAGCGTTCGGGTTGGACG | Reverse | Clone a 367-bp fragment of ropA1 into pJG194 |
oMC305 | CTGGAACCAGGAAGACTTCG | Forward | Detection of integration of pJG581 |
oMC314 | CGCGGATCCGAAGATCTCGAAGGACTGCTC | Forward | Clone a 334-bp fragment of hisC4 into pJG194 |
oMC315 | CGCTCTAGAGATTGCGGATCTTGTCGAAGG | Reverse | Clone a 334-bp fragment of hisC4 into pJG194 |
oMC316 | CGCGGATCCCATGGCTTCCGCAAGGACC | Forward | Clone a 405-bp fragment upstream of ropA1 in pJG194 |
oMC317 | CGCTCTAGACTTGATGTTCATTTCTGACCTCC | Reverse | Clone a 405-bp fragment upstream of ropA1 in pJG194 |
oMC318 | CGCGGATCCGTTCAATTCCGATACGGATTCG | Forward | Clone a 314-bp fragment of ropA2 into pJG194 |
oMC319 | CGCTCTAGACGAGCAGGTCGAAAGTCACG | Reverse | Clone a 314-bp fragment of ropA2 into pJG194 |
oMC320 | CGCAAGCTTGAAGGTCCGAAGCCAGTCG | Forward | Detection of integration of pJG583 |
oMC326 | CCAATATCGCCATCGGAGAG | Forward | Detection of integration of pJG582 |
oMC345 | CGCGGATCCAAGATTGCGGCACGCATCG | Forward | Clone a 320-bp fragment of hisC4 into pJG194 |
oMC346 | CGCTCTAGACATAGGGTACCGTGACCAGC | Reverse | Clone a 320-bp fragment of hisC4 into pJG194 |
oMC347 | AACGTCACAACGCCAAGTGC | Forward | Detection of integration of pJG624 |
oMC354 | CGCGGATCCAACGATGGGCATATGTACC | Forward | Clone a 330-bp fragment upstream of ropA1 in pJG194 |
oMC355 | CGCTCTAGAGGATAAAACCGGGCAAGAGC | Reverse | Clone a 330-bp fragment upstream of ropA1 in pJG194 |
oMC356 | TGACGCGGATCGAATGCAGC | Forward | Detection of integration of pJG627 |
oMC357 | CGCGGATCCGAGCCCATGGAATACGTTCG | Forward | Clone a 319-bp fragment of ropA1 into pJG194 |
oMC358 | CGCTCTAGACTTCATCGACGTCGATCAGG | Reverse | Clone a 319-bp fragment of ropA1 into pJG194 |
oMC359 | GAAGCAAGGGCGGTTGATCG | Forward | Detection of integration of pJG628 |
oMC360 | CGCGGATCCAACCCGAACGCTTACTGG | Forward | Clone a 291-bp fragment of ropA1 into pJG194 |
oMC361 | CGCTCTAGATCAGGTCAGATTAGAAGTCACG | Reverse | Clone a 291-bp fragment of ropA1 into pJG194 |
oMC362 | GCTCGCCTACATCTACGACG | Forward | Detection of integration of pJG629 |
oMC363 | CGCGGATCCGACCATCAACAGGAAGATGG | Forward | Clone a fragment of SMc02397 into pJG194 |
oMC364 | CGCTCTAGACTTTTGCTCTCACCGTAAGCG | Reverse | Clone a fragment of SMc02397 into pJG194 |
oMC365 | GTCAAGGAGACCACGCTTGC | Forward | Detection of integration of pJG630 |
oMC366 | CGCGGATCCGCAGCTACGACACGGAATGG | Forward | Clone a second fragment of SMc02400 into pJG194 |
oMC367 | CGCTCTAGACTGTGTAGTTGATCGCGAAGC | Reverse | Clone a second fragment of SMc02400 into pJG194 |
oMC368 | GCTTCTTCTACAGCTGGTGG | Forward | Detection of integration of pJG631 |
oMC369 | TTTGCGATGCTTTCGGCATGG | Forward | Detection of integration of pJG581 |
oMC370 | CAAGATCGGCGGCTTCATCC | Forward | Detection of integration of pJG584 |
Restriction sites used for cloning are underlined.