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. 2013 Aug;195(16):3663–3671. doi: 10.1128/JB.00480-13

Table 2.

Primers used in this study

Name Sequencea Direction Purpose
oJG664 CAGTTTACTTTGCAGGGCTTCC Forward Sequence verification of pJG194 inserts
oJG1243 TGCGAAAAAGGATGGATATACCG Reverse Sequence verification of pJG194 inserts
oJG524 GGTGGCGCACTTCCTGATAGC Forward Sequence verification of pRF771 inserts
oJG525 CGTTATCAGAACCGCCCAGACC Reverse Sequence verification of pRF771 inserts
oMC023 CGCTCTAGACCCAGACCCGTTTGAAACTTTTG Forward Clone ropA1 into pRF771
oMC024 CGCGGATCCGTAGCCATACTCCAGAAAAGAG Reverse Clone ropA1 into pRF771
oMC029 CGAAAGCCTACGATCACAGG Forward Sequencing of ropA1 mutants
oMC030 CGAAGAAGAGGTGCTGTTCC Reverse Sequencing of ropA1 mutants
oMC303 CGCGGATCCTGAAGCCTACATCCAGCTCG Forward Clone a 367-bp fragment of ropA1 into pJG194
oMC304 CGCTCTAGAGTAAGCGTTCGGGTTGGACG Reverse Clone a 367-bp fragment of ropA1 into pJG194
oMC305 CTGGAACCAGGAAGACTTCG Forward Detection of integration of pJG581
oMC314 CGCGGATCCGAAGATCTCGAAGGACTGCTC Forward Clone a 334-bp fragment of hisC4 into pJG194
oMC315 CGCTCTAGAGATTGCGGATCTTGTCGAAGG Reverse Clone a 334-bp fragment of hisC4 into pJG194
oMC316 CGCGGATCCCATGGCTTCCGCAAGGACC Forward Clone a 405-bp fragment upstream of ropA1 in pJG194
oMC317 CGCTCTAGACTTGATGTTCATTTCTGACCTCC Reverse Clone a 405-bp fragment upstream of ropA1 in pJG194
oMC318 CGCGGATCCGTTCAATTCCGATACGGATTCG Forward Clone a 314-bp fragment of ropA2 into pJG194
oMC319 CGCTCTAGACGAGCAGGTCGAAAGTCACG Reverse Clone a 314-bp fragment of ropA2 into pJG194
oMC320 CGCAAGCTTGAAGGTCCGAAGCCAGTCG Forward Detection of integration of pJG583
oMC326 CCAATATCGCCATCGGAGAG Forward Detection of integration of pJG582
oMC345 CGCGGATCCAAGATTGCGGCACGCATCG Forward Clone a 320-bp fragment of hisC4 into pJG194
oMC346 CGCTCTAGACATAGGGTACCGTGACCAGC Reverse Clone a 320-bp fragment of hisC4 into pJG194
oMC347 AACGTCACAACGCCAAGTGC Forward Detection of integration of pJG624
oMC354 CGCGGATCCAACGATGGGCATATGTACC Forward Clone a 330-bp fragment upstream of ropA1 in pJG194
oMC355 CGCTCTAGAGGATAAAACCGGGCAAGAGC Reverse Clone a 330-bp fragment upstream of ropA1 in pJG194
oMC356 TGACGCGGATCGAATGCAGC Forward Detection of integration of pJG627
oMC357 CGCGGATCCGAGCCCATGGAATACGTTCG Forward Clone a 319-bp fragment of ropA1 into pJG194
oMC358 CGCTCTAGACTTCATCGACGTCGATCAGG Reverse Clone a 319-bp fragment of ropA1 into pJG194
oMC359 GAAGCAAGGGCGGTTGATCG Forward Detection of integration of pJG628
oMC360 CGCGGATCCAACCCGAACGCTTACTGG Forward Clone a 291-bp fragment of ropA1 into pJG194
oMC361 CGCTCTAGATCAGGTCAGATTAGAAGTCACG Reverse Clone a 291-bp fragment of ropA1 into pJG194
oMC362 GCTCGCCTACATCTACGACG Forward Detection of integration of pJG629
oMC363 CGCGGATCCGACCATCAACAGGAAGATGG Forward Clone a fragment of SMc02397 into pJG194
oMC364 CGCTCTAGACTTTTGCTCTCACCGTAAGCG Reverse Clone a fragment of SMc02397 into pJG194
oMC365 GTCAAGGAGACCACGCTTGC Forward Detection of integration of pJG630
oMC366 CGCGGATCCGCAGCTACGACACGGAATGG Forward Clone a second fragment of SMc02400 into pJG194
oMC367 CGCTCTAGACTGTGTAGTTGATCGCGAAGC Reverse Clone a second fragment of SMc02400 into pJG194
oMC368 GCTTCTTCTACAGCTGGTGG Forward Detection of integration of pJG631
oMC369 TTTGCGATGCTTTCGGCATGG Forward Detection of integration of pJG581
oMC370 CAAGATCGGCGGCTTCATCC Forward Detection of integration of pJG584
a

Restriction sites used for cloning are underlined.