Fig 5.

MtvR impacts susceptibility to antibiotics. The role of MtvR in the resistance of the wt and derivative strains overexpressing MtvR (+MtvR) or with MtvR silenced (−MtvR) to the indicated antibiotics was assessed by broth microdilution. Growth inhibition was measured at 590 nm and 640 nm after 24-h static incubation at 37°C. The error bars represent the standard deviation of the mean based on 4 assays with biological triplicates. P values are shown above the corresponding bar groups. The horizontal solid lines crossing the MIC value bars for each antibiotic indicate the threshold MIC value above which strains are considered resistant, according to the CLSI guidelines (30).