Fig 4.

Loss of Skp and BamB results in synthetic LptD, LamB, and FhuA assembly defects. Cultures were grown to an OD₆₀₀ of about 0.75 to 0.9 in LB medium. Samples were analyzed by SDS-PAGE and immunoblotted with anti-LptD, anti-LamB, and anti-FhuA antibodies. “X-Band” is a cross-reacting band of approximately 55 kDa, shown here as a loading control. The ΔsurA strain is shown here as a control. The Δskp bamB::kan and ΔfkpA bamB::kan strains exhibit decreased LptD and FhuA levels compared to those of the single-mutant strains.